The genetically modified flax, event FP967, with tolerance to sulfonylurea
herbicides, is one of the commercial genetically modified events approved in
Canada and USA.



This event is not authorized in Switzerland and EU, therefore, a method to
specifically detect the CDC Triffid line, was required. We revealed the 3′
integration junction sequence between host plant DNA and the integrated gene
construct FP967 by means of Restriction Site PCR and both a qualitative and
a quantitative PCR detection assays were developed. The qualitative PCR
revealed a limit of detection of 0.01% of GM flax in 100 ng of genomic DNA.
The quantitative PCR assay showed a limit of detection of about 9 haploid
genome copies. The specificity and sensitivity of the assay indicate that
the developed event-specific PCR methods can be used for identification and
quantification of FP967 flax.



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