Glutinous cytoplasmic male sterile (CMS) line is vital for the selection of
hybrid glutinous rice combination with high yield and quality. To develop
glutinous CMS with low amylose content, researcher Xin Wang and team knocked
out the granule-bound starch synthase OsWaxy in 209B using CRISPR-Cas9
mediated genome editing technology and successfully obtained a glutinous
maintainer line WX209B.

When compared with maintainer line 209B, WX209B exhibited reduced amylose
contents and similar agronomic characteristics. After one generation of
hybridization and two generations of backcrossing with WX209B as the male
parent and 209A as the female parent, the glutinous CMS line WX209A was
successfully achieved.

The results of the study provide a strategy to efficiently breed for the
glutinous cytoplasmic male sterile line by combining CRISPR-Cas9-mediated
gene editing technology with conventional backcross breeding method in a
short period, which prepares the ground for further breeding of hybrid
glutinous rice variety.



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