Scientists from The University of Tokyo achieved targeted knockout of
mitochondrialgenes
[www.isaaa.org]
Arabidopsis using mitochondria-targeted TALENs (mitoTALENs). The details
of the study are published in/The Plant Journal/.

Using three mitoTALENs promoters and two types of TALENs, the
researchers successfully deleted the two mitochondrial genes (/ATP
synthases 6�?�1/ and/ 6�?�2/) in Arabidopsis. Results showed that the RPS5A
promoter was the most effective among the three and conventional
mitoTALENs were more effective than single�?�molecule mito�?�compact TALENs.
The deletion was achieved by crossing and by floral�?�dip transformation
to introduce the mitoTALENs constructs into the nucleus.

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