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CLAVAMOX SUITABLE AS ANTIBIOTIC FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE CHRYSANTHEMUM 'VIVID SCARLET'
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: August 10, 2014 10:17PM

Genetic transformation requires suitable antibiotics for regeneration of
transformed tissues. A study conducted by Kyungpook National University led
by Chang Kil Kim, examined the effects of antibiotics carbenicillin,
cefotaxime, and Clavamox on in vitro plant regeneration of Chrysanthemum
morifolium 'Vivid Scarlet'.

Shoot induction and the number of shoots per explant were recorded after 5
weeks of culture. Carbenicillin and Clavamox had less inhibitory effects on
number of shoots per explant than cefotaxime. Superior plant growth was
observed in shoots treated with 125 mg/L Clavamox. No variation was observed
in ploidy level between control plants and plants regenerated in vitro with
125 mg/L Clavamox.

These findings suggest that Clavamox can effectively replace carbenicillin
or cefotaxime in Agrobacterium-mediated transformations of 'Vivid Scarlet'.

[www.pomics.com]



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