Transgenic maize event 5307 produces insecticidal protein eCry3.1Ab, an
engineered chimera of Cry1Ab and modified Cry3A, to have internal protection
against Western corn rootworm (Diabrotica virgifera virgifera). Laboratory
tests were conducted to check if maize event 5307 cultivation would affect
non-target organisms such as earthworm, mouse, shrimp, catfish, beetles, and
bugs.

The non-target organisms were fed with eCry3.1Ab or diet with maize tissues
and then evaluated for effects. Results showed that there were no
significant differences in the survival of the non-target organisms exposed
to the insecticidal protein and the control. Measured eCry3.1Ab
concentrations in the laboratory studies were equal to or greater than the
most conservative estimates of environmental exposure. The results of the
laboratory studies indicate that there is no ecological risk in cultivation
of maize event 5307.

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