A new DNA sequencing technique was developed through isolating nuclear DNA
from organelle DNA done by researchers from New England Biolabs and New
Mexico State University. This isolating technique was done by adapting the
procedure used in humans for studying genomics. The process uses
methyl-CpG-binding domain (MBD2) in the genomic DNA (gDNA) obtained from
five angiosperm species methylation followed by sequencing the methyl
enriched sample and methyl depleted sample.
Their findings suggested that the methyl enriched sample gain an increase in
their nuclear DNA and a decrease in mitochondrial DNA (mtDNA) and
chloroplast DNA (cpDNA) by 1.3-29.0 fold and 1.8-31.3 fold, respectively.
The methyl depleted DNA shows an increase in their organelle DNA by 3.2-11.1
fold in cpDNA and 3.4-11.3 fold in mtDNA. This result shows that MBD2 can be
an alternative approach in attaining increase concentrations of nuclear and
organelle DNA for genome sequencing. It also provides a more cost-effective
way for genome sequencing due to its fast and simple techniques applied
without acquiring too much starting material.