In terms of efficiency, targeted DNA integration is better than conventional
means of genetic modificcation which usually results in random insertions.
These specifically integrated transgenes are guaranteed to co-segregate, and
expression levels are more predictable, thus, more manageable.
J.R. Simplot researchers, led by Adrienne Forsyth, now describe a method
that integrates a TALEN-mediated induction of double strand breaks (DSBs)
with a non-autonomous marker selection to insert a transgene into a
selected, active region of the potato (Solanum tuberosum) genome.
The TALEN was designed to create a DSB in the genome sequence following a
constitutive promoter. The donor vector contained the gene of interest
cassette and a promoter-less, plant-derived, herbicide resistance gene to
act as the non-autonomous marker. Transformed potato events occurred with
high frequency, with each of the resulting events consistently expressing
the gene of interest.
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