Wendelin Schnippenkoetter from the CSIRO Agriculture and Food in Australia
investigated the ability of the
[www.isaaa.org] wheat
Lr34 multipathogen resistance
[www.isaaa.org] gene
(Lr34res) to function across a wide range of species using transgenic
sorghum (Sorghum bicolor). The transgenics exhibited increased resistance to
sorghum rust, caused by Puccinia purpurea, and anthracnose disease caused by
Colletotrichum sublineolum.

Furthermore, transgenic sorghum lines highly expressing the wheat Lr34res
gene also exhibited increased immunity to sorghum rust compared to
low-expressing single copy transgenics, which only exhibited partial
resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins
was also evident and characterized highly expressing transgenic lines
inoculated with P. purpurea.

Metabolite analysis of mesocotyls infected with C. sublineolum also showed
increased levels of 3-deoxyanthocyanidin metabolites. These metabolites are
associated with reduced symptoms of anthracnose.

[onlinelibrary.wiley.com]



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