Flowering marks the transition from vegetative growth to reproductive growth
and has considerable effects on
<[www.isaaa.org]
load/biotech-crop-annual-update-soybean-2016.pdf> soybean (Glycine max).
Chinese Academy of Agricultural Sciences researchers, led by Yupeng Cai,
used the CRISPR-Cas9 system to induce targeted mutagenesis of GmFT2a, an
integrator in the photoperiod flowering pathway in soybean.

The soybean cultivar Jack was transformed with three sgRNA-Cas9 vectors
targeting different sites of the GmFT2a. Site-directed mutations were
observed at all targeted sites by DNA sequencing analysis. T1 generation
soybean plants homozygous for null alleles of GmFT2a exhibited late
flowering under natural conditions in Beijing, China. The targeted
mutagenesis was also found to be stably heritable in the following T2
generation.

The team then identified some "transgene-clean" soybean plants that were
homozygous for null alleles of GmFT2a and without any transgenic element
from the T1 and T2 generations. These mutants may provide materials for more
in-depth research of GmFT2a functions.

[onlinelibrary.wiley.com]



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