Epigenetic Basis of High Regeneration Ability of an Elite Cotton Genotype Jin668
The improvement of cotton is highly dependent on efficient tissue culture
and genetic transformation techniques. Somatic regeneration in cotton is
highly genotype-dependent, and a while has gone since a highly regenerative
cotton genotype was developed. Nevertheless, a group of scientists developed
a new elite genotype for cotton (Jin668) through the Successive Regeneration
Acclimation strategy in 2017.
The plant tissue culture process affects the expression of many genes,
leading to epigenetic modifications throughout the regeneration process.
Researchers further explored these epigenetic mechanisms during the
regeneration process of Jin668 through Bisulfite-treated, RNA, and sRNA
sequencing, ChIP assay, and qRT-PCR. The methodology and results of the
study may be used to improve the plant regeneration and transformation
efficiencies for cotton improvement.