Gene Orange (Or) plays an important role in the accumulation of carotenoid
and participates in the non-pigmented plastidation of chromosomes. It does
not interfere with the carotenoid synthesis, but regulates the process of
reservoirs for the isolation and storage of carotenoids. Or gene expression
increased carotenoid accumulation in the scar tissue of rice and sweet
potato. Applying gene technology to express the carotenoid accumulation gene
is an important way to improve carotenoid content, increase the nutritional
value of maize, contribute to human health and effective in breeding.



The research team of Vietnam Academy of Science and Technology has isolated
the Or gene from the Hoang Long sweet potato variety (IbOr) (code
KX792094.1) and the Or gene from cauliflower (code KX396544.1). The team
also designed two expression structures carrying the IbOr gene to enhance
carotenoid accumulation: pCambia2300/Ubi/IbOr/Nos and
pCambia2300/Glo1/IbOr/Nos.



The pCambia2300/Glo1/IbOr/Nos construct was successfully transferred into
several maize lines and developed 18 transgenic lines with a total
carotenoid content increase from 1.756 to 12.515 times, respectively, and
â-carotene increased from 2,022 to 19,002 times. Of these, six lines had
â-carotene levels more than 10 times.

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