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Genetic Transformation of Maize with Drought-responsive OsNAC45 Gene
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: January 30, 2019 01:15AM

Plants respond to abiotic stresses by initiating a series of processes
including the activation of transcription factors that can regulate
expression of various stress-responsive and adaptive genes. NAC family (NAM,
ATAF1/2, CUC2), which is the largest plant transcription factor family,
plays an important role in the development and stress responses of plants.
OsNAC10 gene fragment has1080 nucleotide containing an open reading frame
(OFR) encoding for protein OsNAC45. OsNAC45 is expressed predominantly in
roots and leaves and induced by drought, high salitiny, low temperature, and
abscisic acid. Overexpression of OsNAC45 in rice increased the plant
tolerance to drought and high salinity.

In a study published in Vietnam Journal of Biotechnology, recombinant
expression vector containing OsNAC45-encoding sequence under the control of
the Arabidopsis stress-inducibleRd29Apromoter was designed and successfully
transformed into model maize plants via Agrobacterium tumefaciens bacteria.
The genotype of regeneration plants was identified by PCR and Real-time
pPCR. Using semi-quantitative RT-PCR, the expression of transgene OsNAC45 in
some T0 transgenic plants were detected. These results provide a basis for
the study of the function of OsNAC45 in drought responses and for the
development of drought stress tolerant crops.


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