Crop breeding programs aim to develop pure inbred lines with several
favorable characteristics. Doubled haploid (DH) and genome editing with
CRISPR-Cas9 are two powerful tools in crop breeding. However, both
techniques fall short for rapid development of pure elite lines with
integrated desired traits. Thus, Chinese Academy of Agricultural Sciences
researchers developed a Haploid-Inducer Mediated Genome Editing (IMGE)
approach, which uses a maize haploid inducer line carrying a CRISPR-Cas9
cassette targeting for a desired traits to pollinate an elite maize inbred
line and to generate genome-edited haploids in the elite maize background.
Through this new approach, homozygous pure DH lines with the desired trait
could be generated in just two generations, avoiding the long procedure of
repeated crossing and backcrossing done in conventional breeding.



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