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Transformation of E. coli Modified gdha Gene into Tobacco Improves Productivity and Stress Tolerance
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: July 06, 2019 01:27AM

The bacterial gdhA gene encodes NADH-GDH enzyme. The gdhA-transgenic tobacco
(Nicotiana tabacum) plants exhibited high growth performance and enhanced
herbicide resistance as well as drought tolerance. In addition, the gdhA
gene when expressed in maize plants could increase productivity because of
improving stress tolerance.

In a study published in Academia Journal of Biology, the researchers
isolated and analyzed a gdhA gene from the E. coli strain JM109. Then, the
E.coli derived-gdhA sequence was modified by using OptimumGeneT software for
plant compatibility. The optimized gdhA gene was introduced into the A.
tumefaciens strain EHA105. In order to examine the expression of the gdhA
gene, gdhA-transgenic plants were generated via Agrobacterium-mediated
transformation into tobacco K326. Further analysis showed that two out of 20
plants were positive to the presence of the gdhA gene. These results
suggested that the gdhA expression construction can be transformed into
other crops such as maize.


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