Researchers from Hankyong National University, South Korea, edited three
rice geenes (OsF3$B!l(BH, OsDFR and OsLDOX) involved in anthocyanin production.
Modifying a trait using CRISPR-Cas9 mutagenesis has been proven to provide
advantages in identifying gene function and crop improvement. The results of
the gene editing are published in Plant Biotechnology Reports.
The findings revealed that the ratio of the edited plants in the transformed
early generation was 57 percent. The edited mutant lines were found to
exhibit changes in the seed color and anthocyanin content. Furthermore, all
mutations were stably inherited to the second offspring generation. The
transfer DNA was undetected in the first offspring generation as well as the
backbone sequences in the whole genome resequencing.
Based on the results, CRISPR-Cas9 can be useful in inducing gene-specific
mutations and the mutants are highly similar with non-GMO plants.