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Scientists Use TALENs for Targeted Genome Editing of Potato
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: July 08, 2020 03:04PM

Osaka University and partners reported targeted Genome editing in potato using transcription activator-like effector nucleases (TALENs).

Genome editing using site-specific nucleases such as clustered regularly interspaced short palindromic repeat‚??CRISPR-associated protein 9 and TALENs are highly useful in crop breeding. In the study, the researchers infected potato, with¬†Agrobacterium tumefaciens¬†harboring TALEN-expression vector targeting¬†sterol side chain reductase 2¬†(SSR2) gene and regenerated shoots without selection. This resulted to regenerated lines with disrupted-SSR2¬†gene¬†and without transgene of the TALEN gene, indicating transient gene expression.
Based on the results, Agrobacterial mutagenesis has the potential to accelerate the use of genome-editing technology to modify heterozygous plant genomes.

[www.jstage.jst.go.jp]



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