Osaka University and partners reported targeted¬†Genome editing¬†in potato using transcription activator-like effector nucleases (TALENs).
Genome editing using site-specific nucleases such as clustered regularly interspaced short palindromic repeat‚??CRISPR-associated protein 9 and TALENs are highly useful in crop breeding. In the study, the researchers infected potato, with¬†Agrobacterium tumefaciens¬†harboring TALEN-expression vector targeting¬†sterol side chain reductase 2¬†(SSR2) gene and regenerated shoots without selection. This resulted to regenerated lines with disrupted-SSR2¬†gene¬†and without transgene of the TALEN gene, indicating transient gene expression.
Based on the results, Agrobacterial mutagenesis has the potential to accelerate the use of genome-editing technology to modify heterozygous plant genomes.