Researchers from King Fahd University of Petroleum and Minerals and Texas Tech University reviewed the latest approaches used to minimize off-target effects in genome editing such as biased or unbiased off-target detection, cytosine or adenine base editors, prime editing, dCas9, Cas9 paired nickase, ribonucleoprotein delivery and truncated gRNAs. The review article is published in Cells.
According to the article, CRISPR-Cas9 has been the most popular genome editing tool because of its accuracy, efficiency, cost-effectiveness, and ease of use compared to others. However, CRISPR-Cas9 may cause off-target effects at the genome level and off-target detection tools are also necessary. The most preferred off-target detecting biased and unbiased methods presented were Elevation and GUIDEā??seq, respectively. Furthermore, choosing Cas variants is also critical to reduce the off-target effects that depend on the nature of the experiment.
[www.mdpi.com]