Researchers from Universit?? Paris-Saclay in France reported successful
use of Prime Editing in potato and spreading earthmoss (/Physcomitrium
patens/), a model organism widely used for plant research. Their
findings are published in/Plant Science/.

CRISP-Cas9 technology has revolutionized plant research and precision
crop breeding. Though it has been proven to be an efficient tool for
disruptive targeted mutagenesis, its use in the introduction of precise
and predictable nucleotide substitutions still needs improvement.
However, the development of Prime Editing technology has allowed the
simultaneous generation of nucleotide transitions and transversions as
well as short defined indels.

The research team tested the use of Prime Editing in model organisms
spreading earthmoss and potato, which has a complexgenome. Results
showed that for both plants, the editing rates were lower than with
other CRISPR-Cas9-based techniques, but the researchers were able to
successfully introduce nucleotide transversions into
targetedgenesthrough Prime Editing. Furthermore, the analysis of
potential off-target mutation sites in spreading earthmoss suggested
very high targeting fidelity.

Prime Editing in the model plant Physcomitrium patens and its potential
in the tetraploid potato - ScienceDirect
[www.sciencedirect.com]