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Checkbiotech: Plant gene lends antibiotic resistance to tobacco

Posted by: Prof. Dr. M. Raupp (IP Logged)

Date: August 27, 2005 08:16AM

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www.czu.cz ; www.usab-tm.ro ; www.raupp.info

Introducing antibiotic resistance genes into plant cells allows successful
transformants to be selected on the basis of their survival in a medium
containing a certain antibiotic. However, the source of these genes has
hitherto been bacteria, and some scientists are concerned that horizontal
gene transfer may occur, transferring DNA from plants into microbes, and
resulting in the latter's antibiotic resistance. August 2005

In the latest issue of Nature, however, Ayalew Mentewab and C. Neal Stewart
Jr. of the University of Tennessee report that "Overexpression of an
Arabidopsis thaliana ABC transporter confers kanamycin resistance to
transgenic plants." Their research makes use of a gene derived from
Arabidopsis, called Atwbc19, three times larger than the antibiotic
resistance gene from bacteria.

Scientists transferred the gene cassette into tobacco plant cells, then
subjected these cells to varying levels of kanamycin treatment. They found
that using Atwbc19 alone was enough to make plant cells recover from a
medium containing as much as 200 mg/l kanamycin. The cells were also
resistant to kanamycin treatment alone, and not to other antibiotics such as
geneticin, gentamycin, and streptomycin, whose chemical structures are
similar to kanamycin.

Plants resulting from the transformation developed and grew normally, making
the technique an ideal one for meeting biosafety requirements, since the
gene is derived from a fellow plant species. The researchers propose that
such a technique can be applied to agriculturally important species such as
soybean, cotton, Brassica crops, and tomato, and even forest tree species
such as elms, pines, and spruce.
.

Subscribers to Nature can read more at [www.nature.com]
nbt/journal/vaop/ncurrent/abs/nbt1134.html
[www.nature.com]

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Introducing
antibiotic resistance genes into plant cells allows successful =
transformants
to be selected on the basis of their survival in a medium containing a
certain antibiotic. However, the source of these genes has hitherto =
been
bacteria, and some scientists are concerned that horizontal gene =
transfer may
occur, transferring DNA from plants into microbes, and resulting in =
the
latter's antibiotic resistance. August 2005<o:p></o:p>
</td>
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 <o:p></o:p>
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<td width=3D383 valign=3Dtop style=3D'width:287.6pt;padding:0cm 0cm =
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In the latest issue =
of
Nature, however, Ayalew Mentewab and C. Neal Stewart Jr. of the =
University of
Tennessee report that "Overexpression of an =
Arabidopsis =
thaliana ABC transporter confers kanamycin resistance to transgenic
plants." Their research makes use of a gene derived from =
Arabidopsis,
called Atwbc19, three times larger than the antibiotic resistance gene =
from
bacteria. 
 
Scientists transferred the gene cassette into =
tobacco
plant cells, then subjected these cells to varying levels of kanamycin
treatment. They found that using Atwbc19 alone was enough to make =
plant cells
recover from a medium containing as much as 200 mg/l kanamycin. The =
cells
were also resistant to kanamycin treatment alone, and not to other
antibiotics such as geneticin, gentamycin, and streptomycin, whose =
chemical
structures are similar to kanamycin. 
 
Plants resulting from the transformation =
developed and
grew normally, making the technique an ideal one for meeting biosafety
requirements, since the gene is derived from a fellow plant species. =
The
researchers propose that such a technique can be applied to =
agriculturally
important species such as soybean, cotton, Brassica crops, and tomato, and =
even forest
tree species such as elms, pines, and spruce. 
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