CRISPR-Cas9 has been widely used to produce
gene-specific edits in both mammalian and plant systems. Most modifications
in plants have been small insertions or deletions, with only a few large
target gene deletions, especially for indica rice.
The team of Ying Wang from Syngenta Biotechnology
s/download/Facts%20and%20Trends%20-%20China.pdf> China designed several
CRISPR sgRNAs and successfully deleted DNA fragments in the DENSE AND ERECT
PANICLE 1 (DEP1) gene in the elite Indica rice line IR58025B. The team
achieved up to 2% mutation frequency for a 430 bp target and a 9% frequency
for the 10 kb target among T0 events.
Improvements in yield-related traits, such as dense and erect panicles and
reduced plant height, were observed in dep1 homozygous T0 mutant plants
produced via CRISPR-Cas9.