GMOFORUM.AGROBIOLOGY.EU :  Phorum 5 The fastest message board... ever.
Goto Thread: PreviousNext
Goto: Forum ListMessage ListNew TopicSearchLog In
Downregulation of C3'H Gene in Rice Alters Cell Wall Structures
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: June 26, 2018 08:34AM

p?\Coumaroyl ester 3?\hydroxylase (C3@H) is a key enzyme involved in the
synthesis of lignin, a major component in vascular plants' secondary cell
walls. Although the crucial role of C3@H in have been investigated, there
is limited information available for its role in monocotyledonous grass
species. The team of Yuri Takeda from Kyoto University in Japan studied the
effect of C3@H?\deficiency on the structure and properties of grass cell

C3@H?\knockdown lines generated via RNAi gene silencing, with approximately
0.5% of the normal expression level, reached maturity and set seeds.
Meanwhile, C3@H?\knockout rice mutants generated via CRISPR-Cas9
mutagenesis were severely dwarfed and sterile.

Analysis of the mature C3@H?\knockdown RNAi lines revealed that their
lignin were enriched in p?\hydroxyphenyl (H) units and had reduced levels of
the normally dominant guaiacyl (G) and syringyl (S) units. Suppression of C3
@H also resulted in substantial reduction in cell wall cross?\linking. The
C3@H?\suppressed rice exhibited enhanced biomass saccharification.

These data demonstrate that C3@H expression is an important determinant of
lignin content and composition as well as the degree of cell wall cross?\


Sorry, only registered users may post in this forum.
This forum powered by Phorum.