GMOFORUM.AGROBIOLOGY.EU :  Phorum 5 The fastest message board... ever.
GMO RAUPP.INFO forum provided by WWW.AGROBIOLOGY.EU 
Goto Thread: PreviousNext
Goto: Forum ListMessage ListNew TopicSearchLog In
MitoTALENs Used to Delete Mitochondrial Genes in Arabidopsis
Posted by: Prof. Dr. M. Raupp (IP Logged)
Date: November 09, 2020 04:44PM

Scientists from The University of Tokyo achieved targeted knockout of
mitochondrialgenes
[www.isaaa.org]
Arabidopsis using mitochondria-targeted TALENs (mitoTALENs). The details
of the study are published in/The Plant Journal/.

Using three mitoTALENs promoters and two types of TALENs, the
researchers successfully deleted the two mitochondrial genes (/ATP
synthases 6‚?ź1/¬†and/¬†6‚?ź2/) in Arabidopsis. Results showed that the RPS5A
promoter was the most effective among the three and conventional
mitoTALENs were more effective than single‚?źmolecule mito‚?źcompact TALENs.
The deletion was achieved by crossing and by floral‚?źdip transformation
to introduce the mitoTALENs constructs into the nucleus.

[onlinelibrary.wiley.com]



Sorry, only registered users may post in this forum.
This forum powered by Phorum.