Scientists from The University of Tokyo achieved targeted knockout of
Arabidopsis using mitochondria-targeted TALENs (mitoTALENs). The details
of the study are published in/The Plant Journal/.
Using three mitoTALENs promoters and two types of TALENs, the
researchers successfully deleted the two mitochondrial genes (/ATP
synthases 6‚?ź1/¬†and/¬†6‚?ź2/) in Arabidopsis. Results showed that the RPS5A
promoter was the most effective among the three and conventional
mitoTALENs were more effective than single‚?źmolecule mito‚?źcompact TALENs.
The deletion was achieved by crossing and by floral‚?źdip transformation
to introduce the mitoTALENs constructs into the nucleus.