I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape
Researchers from France evaluated the use of two meganucleases, I-/Sce/I
and a customized meganuclease, in tomato and oilseed rape. Their
findings are published in/Transgenic Research/.
Meganucleases are molecular scissors that can cause changes in the DNA.
Because of this function, they are used in editing the genomesof plants.
To assess their efficacy in tomato and oilseed rape, the researchers
employed different strategies.
First, they used a reporter construct GFFP with the target sequences
which showed that both meganucleases can generate double-strand break
and HDR mediated recombination in a reporter gene. They also observed
that I-/Sce/I have a higher DSB efficiency than the customized
meganuclease. DSB efficiency reached up to 62.5% in tomato and 44.8% in
For the second strategy, the researchers introduced the same exogenous
landing pad for tomato and oilseed rape. I-/Sce/I turned out to be less
efficient, while the customized meganuclease generated the excision of
an exogenous transgene present in tomato.
I-SceI and customized meganucleases-mediated genome editing in tomato
and oilseed rape | SpringerLink