The sea-island cotton GbTCP4 transcription factor positively regulates drought and salt stress responses
TCP transcription factors play important regulatory roles in plantgrowth
however, their function in response to salt and drought stress in
sea-island cotton (/Gossypium barbadense
Here,/GbTCP4/expression was induced byabscisic acid
drought, and NaCl treatments. Under drought stress, compared to
root length and survival rate; additionally, it was ABA-insensitive at
the germination stage but ABA-sensitive at the seedling stage, showing
reduced stomatal opening and ABA enrichment.
Under salt stress, compared to WT/Arabidopsis/,
transgenic/GbTCP4-/overexpressing/Arabidopsis/showed greater root
length, survival rate, and SPAD value and lower malondialdehyde (MDA)
content. Conversely, under drought or salt stress, virus-induced
gene-silenced/GbTCP4/cotton showed decreased root length, area and
volume and increased MDA content and sensitivity to drought and salt
stress compared with control cotton.
RNA-seq and quantitative real-time PCR analyses showed
that/GbTCP4/affected the transcription levels of genes across multiple
abiotic stress-related metabolic pathways. Furthermore, GbTCP4 activated
the transcription of/GbUVR8/and/GbbHLH130/by binding to their promoters.
These results suggest that GbTCP4 positively regulates drought and salt
stress responses and is a suitable candidate gene for improving plant
drought and salt tolerance.